This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.9 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human B7-H3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human B7-H3, Fc Tag (Cat. No. B73-H5253) is more than 85% and the molecular weight of this protein is around 105-128 kDa verified by SEC-MALS.
Authors: Du H, et al
Journal: Cancer Cell 2019
Application: Stimulation of B7-H3 CAR-Ts
Authors: Gong B, et al
Journal: J Exp Med 2019
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