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Your Position: Home > Immune Checkpoint Proteins > PD-1 > Rat PD-1 / PDCD1 Protein, His Tag

Rat PD-1 / PDCD1 Protein, His Tag

  • Synonym
    PDCD1,PD1,CD279,SLEB2
  • Source
    Rat PD-1, His Tag (PD1-R52H2) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # NP_001100397.1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Gln 167) NP_001100397.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.1 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat PD-1, His Tag (Cat. No. PD1-R52H2) SDS-PAGE gel

Rat PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Rat PD-1, His TagRat PD-1, His Tag (Cat. No. PD1-R52H2) ELISA bioactivity

Immobilized Rat PD-1, His Tag (Cat. No. PD1-R52H2) at 2 μg/mL (100 μL/well) can bind Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. PD1-M52A2) with a linear range of 0.02-0.313 μg/mL (QC tested).

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