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Your Position: Home > Immune Checkpoint Proteins > Nectin-3 > PV3-H52E4

Human Nectin-3 / CD113 Protein, His Tag (MALS verified)

  • Synonym
  • Source
    Human Nectin-3, His Tag (PV3-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3).
    Predicted N-terminus: Gly 58
  • Molecular Characterization
    Online(Gly 58 - Asp 400) Q9NQS3

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.6 kDa. The protein migrates as 55-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human Nectin-3, His Tag (Cat. No. PV3-H52E4) SDS-PAGE gel

Human Nectin-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human Nectin-3, His Tag (Cat. No. ) MALS images

The purity of Human Nectin-3, His Tag(Cat. No. PV3-H52E4) is more than 85% and the molecular weight of this protein is around 65-90 kDa verified by SEC-MALS.

Human Nectin-3, His TagHuman Nectin-3, His Tag (Cat. No. PV3-H52E4) ELISA bioactivity

Immobilized Human Nectin-3, His Tag (Cat. No. PV3-H52E4) at 2 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 1-20 ng/mL (QC tested).


Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-3, His Tag (Cat. No. PV3-H52E4) with an affinity constant of 2.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).


Loaded Human Nectin-3, His Tag (Cat. No. PV3-H52E4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 4.99 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. Nectins are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin system in epithelial cells. PVRL3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Furthermore, PVRL3 induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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