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Your Position: Home > Protein > IL23A & IL12B > ILB-HM52W6

Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (MALS verified)

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  • Synonym
    IL-23 alpha & IL-12 beta
  • Source
    Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free(ILB-HM52W6) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9NPF7-1 (IL23A) & P43432-1 (IL12B)).
    Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
  • Molecular Characterization
    IL23A & IL12B Structure

    Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The protein migrates as 20 kDa (IL23A) & 45 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL23A & IL12B SDS-PAGE

Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL23A & IL12B MALS images

The purity of Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL23A & IL12B ELISA

Immobilized Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5-78 ng/mL (QC tested).

Bioactivity-BLI
 IL23A & IL12B BLI

Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Human IL-23 alpha&Mouse IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 15.7 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

 IL23A & IL12B BLI

Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) with an affinity constant of 2.79 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 IL23A & IL12B CELL

Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.0069-0.0426 ng/mL (Routinely tested).

 IL23A & IL12B CELL

Cell based assay shows that the secretion of IL-17 induced by Human IL-23A & Mouse IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HM52W6) is inhibited by increasing concentration of the Anti-Human IL-23 neutralizing antibody. The IC50 is between 0.41-1.012 ng/mL (Routinely tested).

  • Background
    Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:15 Details
  • Latest Research Phase:Approved

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