0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-H52W9

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)

  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (CDG-H52W9) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # P07766-1(CD3E) & P09693-1(CD3G)).
    Predicted N-terminus: Asp 23 (CD3E) & Gln 23 (CD3G)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) P07766-1 (CD3E) & P09693-1 (CD3G)

    Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 39.2 kDa (CD3E) and 38.6 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag, and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus, followed by a Flag tag. The predicted N-terminus is Asp 23 (CD3E) & Gln 23 (CD3G). The reducing (R) heterodimer protein migrates as 40-50 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. CDG-H52W9) SDS-PAGE gel

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. ) MALS images

The purity of Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) was more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified)Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 2 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.4-6 ng/mL (QC tested).

Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified)Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag(MALS verified) (Cat. No. CDG-H52W9) ELISA bioactivity

Immobilized Human CD3E&CD3G Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with a linear range of 0.4-6 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a)) with an affinity constant of 39.6 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Human CD3 antibody (UCHT1) with an affinity constant of 1.59 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (Cat. No. CDG-H52W9) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Bispecific T-cell Engager (CD3 X BCMA) with an affinity constant of 1.13 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Comments (0)

This web search service is supported by Google Inc.

totop