Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 19 kDa (IL-23A) and 40-44 kDa (IL-12B) respectively due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Loaded Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 20.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 11.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 3.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.008-0.0134 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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