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Your Position: Home > Cytokines > IL23A & IL12B > Mouse IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free

Mouse IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
IL-23 alpha & IL-12 beta
Source
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-M52W7) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14 (IL23A) & NP_032378 (IL12B)).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequence
Molecular Characterization
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 21 kDa (IL-23A) and 45-50 kDa (IL-12B) respectively due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA

Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag FreeMouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) ELISA bioactivity

Immobilized Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) at 10 μg/mL (100 μL/well) can bind Human IL23R with a linear range of 0.078-1.25 μg/mL (Routinely tested).

Protocol

Bioactivity-Cell based assay

Human FcRn Heterodimer Protein Cell_Base

Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.008-0.0134 ng/mL (Routinely tested).

Protocol

Background
Interleukin-23 subunit alpha (IL-23 alpha) can associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
References
- (1) Oppmann B., et al., 2000, Immunity 13:715-725.
- (2) Parham C., et al., 2002, J. Immunol. 168:5699-5708.
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.