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Product Details
Synonyms
TNFRSF4, OX40, CD134, OX40L receptor, ACT35, TXGP1L
Source
Rat OX40, Fc Tag (OX0-R5253) is expressed from human 293 cells (HEK293). It contains AA Val 20 - Pro 210 (Accession # P15725).
Predicted N-terminus: Val 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 47.7 kDa. The protein migrates as 66 kDa under reducing (R) condition, and 150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rat OX40, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Rat OX40, Fc Tag (Cat. No. OX0-R5253) at 2 μg/mL (100 μL/well) can bind Rat OX40 Ligand, His Tag (Cat. No. OXL-R5248) with a linear range of 10-78 ng/mL (Routinely tested).
Protocol
Immobilized Rat OX40 Ligand, His Tag (Cat. No. OXL-R5248) at 2 μg/mL (100 μL/well) can bind Rat OX40, Fc Tag (Cat. No. OX0-R5253) with a linear range of 0.1-1 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 4
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
29 Details
- Highest Development Stage：
Phase 3 Clinical
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