Product Details
Synonyms
PECAM1, CD31, FLJ34100, FLJ58394
Source
Human CD31, Fc Tag (CD1-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Lys 601 (Accession # AAH22512.1).
Predicted N-terminus: Gln 28
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 90.6 kDa. The protein migrates as 120-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human CD31, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Customer Reviews Writing Reviews
J***y2021-10-26Likes
Amazing customer service! Very prompt and received replies from multiple sources. Detailed CoA, which was highly appreciated. Used this protein to attach CD31 to Protein G dynabeads. Shipping was faster than anticipated and came packaged very nicely. I imagine this product could also be adhered to plates with high surface binding chemistry for use in assays where you measure cells adhering to CD31.
Background
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