Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Siglec-3, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human Siglec-3, Avitag,His Tag (Cat. No. CD3-H82E7) is more than 90% and the molecular weight of this protein is around 70-80 kDa verified by SEC-MALS.
2e5 of anti-Siglec-3 CAR-293 cells were stained with 100 μL of 3 μg/mL of Human Siglec-3, Avitag,His Tag (Cat. No. CD3-H82E7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Systematic preclinical evaluation of CD33-directed chimeric antigen receptor T cell immunotherapy for acute myeloid leukemia defines optimized construct design
Authors: Qin H, Yang L, Chukinas JA, et al.
Journal: Immunother Cancer 2021
Application: Flow cytometry
Evidence for C1q-mediated crosslinking of CD33/LAIR-1 inhibitory immunoreceptors and biological control of CD33/LAIR-1 expression
Authors: Son, Myoungsun et al.
Journal: Scientific reports 2021
Application: Slot Blot Assay
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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