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Your Position: Home > Immune Checkpoint Proteins > CD40 > CD0-H82E8

Biotinylated Human CD40 / TNFRSF5 Protein, Avitag™,His Tag (MALS verified)

  • Synonym
    CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50
  • Source
    Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (CD0-H82E8) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Arg 193) P25942-1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No. CD0-H82E8) SDS-PAGE gel

Biotinylated Human CD40, Avitag™,His Tag (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No. ) MALS images

The purity of Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No.CD0-H82E8) is more than 90% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD40, Avitag™,His Tag (MALS verified)Biotinylated Human CD40, Avitag™,His Tag (MALS verified) (Cat. No. CD0-H82E8) ELISA bioactivity

Immobilized Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) at 1 μg/mL (100 μL/well) on Recombinant Streptavidin (Cat. No. STN-N5116) precoated (0.5μg/well) plate, can bind Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) (Cat. No. CDL-H5269) with a linear range of 0.2-2 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Biotinylated Human CD40, Avitag,His Tag (Cat. No. CD0-H82E8) can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 3 μg/mL (Routinely tested).

  • Background
    CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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