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Product Details
Synonyms
TNFSF13B, BAFF, BLYS, CD257, DTL, TALL1, THANK, TNFSF20, ZTNF4, TALL-1
Source
Biotinylated Human BAFF Protein, Avitag,Fc Tag (BAF-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1).
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 44.9 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human BAFF Protein, Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) is more than 85% and the molecular weight of this protein is around 280-320 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human BCMA, Fc Tag (Cat. No. BC7-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) with a linear range of 1.95-15.6 ng/mL (QC tested).
Protocol
Immobilized Human TACI Protein, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) with a linear range of 0.2-6 ng/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR Protein, Llama IgG2b Fc Tag (Cat. No. BAR-H5258): Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8129 μg/mL (Routinely tested).
Protocol
Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag: Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.4692 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Immobilized Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Chip can bind Human TACI Protein, Fc Tag with an affinity constant of 0.988 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Immobilized Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Chip can bind Human BAFFR, Fc Tag with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human BAFF Protein, Avitag,Fc Tag (Cat. No. BAF-H82F3) on SA Biosensor, can bind Human BCMA, His Tag (Cat. No. BCA-H522y) with an affinity constant of 1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
B-cell-activating factor/B lymphocyte stimulator
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Approved
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