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Your Position: Home > Immune Checkpoint Proteins > CD155 > Biotinylated Human CD155 / PVR Protein, Fc Tag, Avi Tag

Biotinylated Human CD155 / PVR Protein, Fc Tag, Avi Tag (Avitag™)

  • Synonym
    PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
  • Source
    Biotinylated Human CD155, Fc Tag (CD5-H82F6) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # AAH15542).
    Predicted N-terminus: Trp 21
  • Molecular Characterization
    Online(Trp 21 - Asn 343) AAH15542

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 63.5 kDa. As a result of glycosylation, the protein migrates as 75-100 kDa under reducing (R) condition, and 140-180 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) SDS-PAGE gel

Biotinylated Human CD155, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human CD155, Fc TagBiotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) ELISA bioactivity

Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) with a linear range of 0.02-0.15 μg/mL (QC tested).

Biotinylated Human CD155, Fc TagBiotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) ELISA bioactivity

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD155, Fc Tag (Cat. No. CD5-H82F6) with a linear range of 0.02-0.08 μg/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Biotinylated Human CD155, Fc Tag, Avi Tag (Cat. No. CD5-H82F6) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/ml (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Biotinylated Human CD155, Fc Tag, Avi Tag (Cat. No. CD5-H82F6) to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/ml. The IC50 is 0.201 μg/ml (Routinely tested).

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Price(USD) : $1380.00

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