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Your Position: Home > Protein > DNAM-1 > DN1-H82F9

Biotinylated Human DNAM-1 / CD226 Protein, Fc,Avitag™

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  • Synonym
    DNAM1,CD226,PTA1
  • Source
    Biotinylated Human DNAM-1, Fc,Avitag(DN1-H82F9) is expressed from human 293 cells (HEK293). It contains AA Glu 19 - Asn 247 (Accession # NP_006557 ).
    Predicted N-terminus: Glu 19
  • Molecular Characterization
    DNAM-1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 54.4 kDa. The protein migrates as 66-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DNAM-1 SDS-PAGE

Biotinylated Human DNAM-1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 DNAM-1 ELISA

Immobilized Rhesus macaque CD155, His Tag (Cat. No. CD5-R52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) with a linear range of 10-156 ng/mL (QC tested).

Bioactivity-FACS
 DNAM-1 FACS

FACS assay shows Biotinylated Human DNAM-1, Fc,Avitag (Cat. No. DN1-H82F9) can bind to 293T cell overexpressing human CD155. The concentration of DNAM-1 is 0.03 μg/mL (Routinely tested).

  • Background
    DX accessory molecule 1 (DM-1), a single-pass type I membrane protein, is also known as CD226 antigen and platelet and T cell activation antigen 1 (PTA1), which contains 2 Ig-like C2-type (immunoglobulin-like) domains. DM-1 is a ~65 kDa glycoprotein expressed on the surface of natural killer cells, platelets, monocytes and a subset of T cells. DM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DM-1 with antibodies causes cellular activation. Furthermore, DM-1 can interact with PVR and PVRL2.
  • Clinical and Translational Updates

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