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Your Position: Home > Bio-Markers & CD Antigens > Glypican 3 > Cynomolgus Glypican 3 / GPC3 Protein, Fc Tag

Cynomolgus Glypican 3 / GPC3 Protein, Fc Tag

  • Synonym
    GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
  • Source
    Cynomolgus Glypican 3 / GPC3, Fc Tag (GP3-C5256) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # XP_005594665).
    Predicted N-terminus: Gln 25
  • Molecular Characterization
    Glypican 3(Gln 25 - His 559) XP_005594665

    This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.5 kDa. GP3-C5256 may be cleaved into secrete GPC3, short propeptide (aa ?-559) & Fc tag, long propeptide (aa ?-559) & Fc tag. The protein migrates as 100 kDa (secrete GPC3 & propeptide & Fc tag), 58 kDa (long propeptide & Fc tag), 40 kDa (short propeptide & Fc tag) and 35 kDa Human IgG1 Fc fragment on a SDS-PAGE gel under reducing (R) condition.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus Glypican 3 / GPC3, Fc Tag (Cat. No. GP3-C5256) SDS-PAGE gel

Cynomolgus Glypican 3 / GPC3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Cynomolgus Glypican 3 / GPC3, Fc TagCynomolgus Glypican 3 / GPC3, Fc Tag (Cat. No. GP3-C5256) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 2μg/mL (100 μL/well) can bind Cynomolgus Glypican 3, Fc Tag (Cat. No. GP3-C5256) with a linear range of 4.8-19.5 ng/mL (QC tested).

  • Background
    Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $290.00

Price(USD) : $1800.00

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