This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 87.2 kDa. GP3-M5254 may be cleaved into secrete GPC3, short propeptide (aa ?-557) & Fc tag, long propeptide (aa 358-557 predicted) & Fc tag. The protein migrates as 100 kDa (secrete GPC3 & propeptide & Fc tag), 58 kDa (long propeptide & Fc tag), 40 kDa (short propeptide & Fc tag) on a SDS-PAGE gel under reducing (R) condition.
>90% GPC3 and 2-8% human IgG1 Fc fragment as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
Mouse Glypican 3 / GPC3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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