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Your Position: Home > Cytokines > Gremlin > GR1-H5254

Human Gremlin / GREM1 Protein, Fc Tag

  • Synonym
    GREM1,CKTSF1B1,DAND2,DRM,PIG2
  • Source
    Human Gremlin, Fc Tag (GR1-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 25 - Asp 184 (Accession # NP_037504).
    Predicted N-terminus: Lys 25
  • Molecular Characterization
    Online(Lys 25 - Asp 184) NP_037504

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 44.2 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with 1 mM EDTA. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human Gremlin, Fc Tag (Cat. No. GR1-H5254) SDS-PAGE gel

Human Gremlin, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Gremlin, Fc TagHuman Gremlin, Fc Tag (Cat. No. GR1-H5254) ELISA bioactivity

Immobilized Human BMP-2, Tag Free (Cat. No. BM2-H4117) at 2μg/mL (100 μL/well) can bind Human Gremlin, Fc Tag (Cat. No. GR1-H5254) with a linear range of 0.8-80 ng/mL (QC tested).

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Price(USD) : $2100.00

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Datasheet & Documentation

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DMF (Drug Master File)

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