>Immune Checkpoint Proteins
>Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human TIGIT, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human CD112, Fc Tag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.
FACS assay shows that recombinant Human TIGIT Protein, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/ml (Routinely tested).
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Price(USD) : $350.00
Price(USD) : $2100.00
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