This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5 μg/mL (100 μL/well) can bind Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TIT-H5253) with a linear range of 0.5-8 ng/mL (QC tested).
Serial dilutions of TIGIT antibody antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.
Loaded Human TIGIT, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TIT-H5253) on Protein A Biosensor, can bind Human CD155, His Tag (Cat. No. CD5-H5223) with an affinity constant of 0.92 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that Human TIGIT, Mouse IgG2a Fc tag, low endotoxin (Cat. No. TIT-H5253) can bind to 293T cell overexpressing human CD155. The concentration of TIGIT used is 0.03 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $2310.00
high quality 2019-nCoV full length S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
CD24 protein with different tags and CD24 overexpressed cells, The binding activity of CD24 overexpressed cells and Siglec-10 was verified by FACS.
Enzyme activity＞37000 pmol/min/μg. Purity is greater than 90%.
Bioactivity is verified by BLI/SPR/ELISA.
HEK293 expressed full length CD20 protein with Nanodisc/DDM. Complete conformation.High affinity(nM) of RTX/OFA and CD20 protein.
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