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Your Position: Home > Immune Checkpoint Proteins > TIGIT > Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin

Human TIGIT Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
  • Source
    Human TIGIT, mouse IgG2a Fc tag (TIT-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Pro 141 (Accession # AAI01290).
    Predicted N-terminus: Met 22
  • Molecular Characterization
    TIGIT(Met 22 - Pro 141) AAI01290

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 40.1 kDa. The protein migrates as 42-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human TIGIT, mouse IgG2a Fc tag (Cat. No. TIT-H5253) SDS-PAGE gel

Human TIGIT, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human TIGIT, mouse IgG2a Fc tagHuman TIGIT, mouse IgG2a Fc tag (Cat. No. TIT-H5253) ELISA bioactivity

Immobilized Human CD155, Fc Tag (Cat. No. CD5-H5251) at 5μg/mL (100 μL/well) can bind Human TIGIT, mouse IgG2a Fc tag (Cat. No. TIT-H5253) with a linear range of 3.9-62.5 ng/mL (QC tested).

  • Background
    TIGIT (also called T cell immunoreceptor with Ig and ITIM domains) is one newly discovered immune receptor on some percentage of T cells and Natural Killer Cells(NK). It is also identified as WUCAM and Vstm3. TIGIT could bind to CD155(PVR) on dendritic cells(DCs), macrophages, etc. with high affinity, and also to CD112(PVRL2) with lower affinity. TIGIT expressing CD8+ T cells has been shown to be expanded and associated with clinical markers of HIV disease progression in a diverse group of HIV infected individuals. TIGIT and PD-1 has been shown to be over expressed on tumor antigen-sepcific (TA-specific) CD8+ T cells and CD8+ tumor infiltrating lymphocytes (TILs) from individuals with melanoma. Blockade of TIGIT and PD-1 led to increased cell proliferation, cytokine production, and degranulation of TA-specific CD8+ T cells and TIL CD8+ T cells.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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