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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > Cynomolgus LAG-3 / CD223 Protein, Fc Tag

Cynomolgus LAG-3 / CD223 Protein, Fc Tag

  • Synonym
    LAG3,CD223,FDC
  • Source
    Cynomolgus LAG-3, Fc Tag (LA3-C5252) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # XP_005570011.1).
    Predicted N-terminus: Ala 18
  • Molecular Characterization
    LAG-3(Ala 18 - His 449) XP_005570011.1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 73.0 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) SDS-PAGE gel

Cynomolgus LAG-3, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.

Bioactivity-ELISA
Cynomolgus LAG-3, Fc TagCynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) ELISA bioactivity

Immobilized Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 8-31 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

Flow Cytometry assay shows that Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252) can bind to daudi cell surface. The concentration of  LAG-3 used is 1 μg/ml (Routinely tested).

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