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Your Position: Home > Immune Checkpoint Proteins > OX40 > Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, His Tag, Avi Tag

Biotinylated Human OX40 / TNFRSF4 / CD134 Protein (recommended for biopanning), Avi Tag (Avitag™)

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Biotinylated Human OX40, His Tag (TN4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318.1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    Online(Leu 29 - Ala 216) NP_003318.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 22.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human OX40, His Tag (Cat. No. TN4-H82E4) SDS-PAGE gel

Biotinylated Human OX40, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human OX40, His TagBiotinylated Human OX40, His Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Anti-OX40 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) with a linear range of 1-5 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Biotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.1 μg/ml (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $1380.00

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