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Your Position: Home > Immune Checkpoint Proteins > OX40 > Biotinylated Human OX40 / TNFRSF4 / CD134 Protein, His Tag, Avi Tag

Biotinylated Human OX40 / TNFRSF4 / CD134 Protein (recommended for biopanning), Avi Tag (Avitag™)

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Biotinylated Human OX40, His Tag, Avi Tag (TN4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # NP_003318.1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40(Leu 29 - Ala 216) NP_003318.1

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 22.8 kDa. The protein migrates as 40-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) SDS-PAGE gel

Biotinylated Human OX40, His Tag, Avi Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human OX40, His Tag, Avi TagBiotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) ELISA bioactivity

Immobilized Anti-OX40 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) with a linear range of 1-5 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Biotinylated Human OX40, His Tag, Avi Tag (Cat. No. TN4-H82E4) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.1 μg/ml (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) is also known as ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, CD antigen CD134, OX40. OX40 / TNFRSF4 contains four TNFR-Cys repeats. TNFRSF4 is receptor for TNFSF4 / OX40L / GP34 and can interacts with TRAF2, TRAF3 and TRAF5.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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