Mouse Glypican 3 / GPC3 Protein

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1
Source
Mouse Glypican 3 / GPC3, His Tag (GP3-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Met 557 (Accession # Q8CFZ4-1).
Predicted N-terminus: Gln 25
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Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 62.4 kDa. The protein migrates as 66-150 kDa, 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Mouse Glypican 3 / GPC3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 0.5 μg/mL (100 μL/well) can bind Mouse Glypican 3, His Tag (Cat. No. GP3-M52H3) with a linear range of 1-16 ng/mL (QC tested).

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Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.
References
- (1) Davoodi J., et al., 2007, Proteomics 7:2300-2310.
- (2) Veugelers M., et al., 2000, Hum. Mol. Genet. 9:1321-1328.
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