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Product Details
Synonyms
LAG3, CD223, FDC
Source
Cynomolgus LAG-3, Mouse IgG2a Fc tag (LA3-C52A0) is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # XP_005570011.1).
Predicted N-terminus: Ala 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 73.5 kDa. The protein migrates as 95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus LAG-3, Mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Cynomolgus LAG-3, Mouse IgG2a Fc tag (Cat. No. LA3-C52A0) was greater than 85% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Cynomolgus LAG-3, Mouse IgG2a Fc tag (Cat. No. LA3-C52A0) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Cynomolgus LAG-3, Mouse IgG2a Fc tag (Cat. No. LA3-C52A0) at 5 μg/mL, add increasing concentrations of Anti-PD1 x LAG3 Antibody and then add Biotinylated Cynomolgus PD-1 Protein, Fc,Avitag (Cat. No. PD1-C82F7) at 2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 0.002 μg/mL (Routinely tested).
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Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte activation gene 3 protein
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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