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Your Position: Home > All Other Proteins > BAFFR > BAR-H5257

Human BAFFR / TNFRSF13C Protein, Fc Tag

  • Synonym
    BAFFR,TNFRSF13C,BROMIX,CD268,CVID4,prolixin,BAFF-R
  • Source
    Human BAFFR, Fc Tag (BAR-H5257) is expressed from human 293 cells (HEK293). It contains AA Ser 7 - Ala 71 (Accession # Q96RJ3-1).
    Predicted N-terminus: Ser 7
  • Molecular Characterization
    Online(Ser 7 - Ala 71) Q96RJ3-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 33.2 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BAFFR, Fc Tag (Cat. No. BAR-H5257) SDS-PAGE gel

Human BAFFR, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human BAFFR, Fc TagHuman BAFFR, Fc Tag (Cat. No. BAR-H5257) ELISA bioactivity

Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 5 μg/mL (100 μL/well) can bind Human BAFFR, Fc Tag (Cat. No. BAR-H5257) with a linear range of 2-31 ng/mL (QC tested).

Human BAFFR, Fc TagHuman BAFFR, Fc Tag (Cat. No. BAR-H5257) ELISA bioactivity

Immobilized Human BAFFR, Fc Tag (Cat. No. BAR-H5257) at 1 μg/mL (100 μL/well) can bind Anti-BAFFR Antibody, Human IgG1 with a linear range of 0.2-5 ng/mL (QC tested).

Human BAFFR, Fc TagHuman BAFFR, Fc Tag (Cat. No. BAR-H5257) ELISA bioactivity

Serial dilutions of Anti-BAFFR Antibody, Human IgG1 were added into Human BAFFR, Fc Tag (Cat. No. BAR-H5257): Biotinylated Human BAFF, His,Avitag (active trimer) (MALS verified) (Cat. No. BAF-H82Q2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.7363 μg/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Immobilized Biotinylated Human BAFF, Avitag,Fc Tag (active trimer) (MALS verified) (Cat. No. BAF-H82F3) on SA Chip can bind Human BAFFR, Fc Tag (Cat. No. BAR-H5257) with an affinity constant of 26.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2300.00

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  • Number of Drugs in Clinical Trials:2 Details
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