Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 65.8 kDa. The protein migrates as 75-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Supplied as 0.2 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human SIRP alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) is more than 90% and the molecular weight of this protein is around 155-170 kDa verified by SEC-MALS.
Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) with a linear range of 0.05-0.2 μg/mL (QC tested).
Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human CD47, Fc Tag (Cat. No. CD7-H5256): Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5431 μg/mL (Routinely tested).
FACS assay shows that Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 3 μg/mL (QC tested).
FACS analysis shows that the binding of Biotinylated Human SIRP alpha, Fc,Avitag (Cat. No. CDA-H82F2) to Jurkat expressing CD47 was inhibited by increasing concentration of neutralizing Anti- Human CD47 antibody. The concentration of SIRP alpha used is 1 μg/mL. IC50=0.1303 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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