Save Product
Product Details
Synonyms
CD112, Nectin-2, PVRL2
Source
Biotinylated Human Nectin-2, Fc,Avitag (CD2-H82F8) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
Predicted N-terminus: Gln 32
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 64.0 kDa. The protein migrates as 66 kDa under reducing (R) condition, and 130 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human Nectin-2, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) is more than 90% and the molecular weight of this protein is around 250-290 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human PVRIG, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-13 ng/mL (QC tested).
Protocol
Immobilized Human PVRIG, Fc Tag (Cat. No. PVG-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) with a linear range of 0.2-16 ng/mL (Routinely tested).
Protocol
Serial dilutions of TIGIT antibody (1:2 serial dilutions, from 20 μg/mL to 0.0097 μg/mL) were added into Human TIGIT, Mouse IgG2a Fc Tag (Cat. No. TIT-H5253): Biotinylated Human Nectin-2, Fc,Avitag (Cat. No. CD2-H82F8) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points before curve fitting.
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
