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Your Position: Home > All Other Proteins > Integrin alpha 6 beta 1 > IT1-H52W7

Human Integrin alpha 6 beta 1 (ITGA6&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha 6 beta 1,ITGA6&ITGB1
  • Source
    Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1) (Accession # P23229-2 (ITGA6) & P05556-1 (ITGB1)).
    Predicted N-terminus: Phe 24 (ITGA6) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 24 - Gly 1012 (ITGA6) & Gln 21 - Asp 728 (ITGB1)) P23229-2 (ITGA6) & P05556-1 (ITGB1)

    Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA6 and ITGB1, has a calculated MW of 116.8 kDa (ITGA6) and 83.7 kDa (ITGB1). Subunit ITGA6 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 140-150 kDa (ITGA6) and 100-118 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) SDS-PAGE gel

Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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