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Your Position: Home > Immune Checkpoint Proteins > LAG-3 > Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag

Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag

  • Synonym
    LAG3,CD223,FDC
  • Source
    Human LAG-3 / CD223, Mouse IgG2a Fc Tag (LA3-H52Aa) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    LAG-3(Leu 23 - Leu 450) P18627-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 73.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human LAG-3 / CD223, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) SDS-PAGE gel

Human LAG-3 / CD223, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human LAG-3 / CD223, Mouse IgG2a Fc TagHuman LAG-3 / CD223, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) ELISA bioactivity

Immobilized Anti-Human LAG3 MAb, Human IgG1 at 0.5 μg/mL (100 μL/well) can bind Human LAG-3, mouse IgG2a Fc tag (Cat. No. LA3-H52Aa) with a linear range of 0.8-3 ng/mL (QC tested).

Human FcRn Heterodimer Protein Cell_Base

Flow Cytometry assay shows that recombinant human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa ) can bind to daudi cell surface. The concentration of LAG3 mFc used is 0.3ug/ml (Routinely tested).

Human FcRn Heterodimer Protein Cell_Base

FACS analysis shows that the binding Human LAG3, Mouse IgG2a Fc Tag to daudi cells was inhibited by increasing concentration of neutralizing anti-LAG3 antibody. The concentration of LAG3 mFc used is 1ug/ml. The IC50 is 0.165ug/ml (Routinely tested).

  • Background
    Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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