>Immune Checkpoint Proteins
>Human LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 73.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
Human LAG-3, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) at 2 μg/mL (100 μL/well) can bind Anti-Human LAG3 MAb, Human IgG1 with a linear range of 1-10 ng/mL (QC tested).
Flow Cytometry assay shows that recombinant human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa ) can bind to daudi cell surface. The concentration of LAG3 mFc used is 0.3μg/ml (Routinely tested).
FACS analysis shows that the binding Human LAG3, Mouse IgG2a Fc Tag to daudi cells was inhibited by increasing concentration of neutralizing anti-LAG3 antibody. The concentration of LAG3 mFc used is 1 μg/ml. The IC50 is 0.165 μg/ml (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $2310.00
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