This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 70.5 kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human EGF R, His Tag (Cat. No. EGR-H5222) is more than 90% and the molecular weight of this protein is around 80-105 kDa verified by SEC-MALS.
Immobilized Human Anti-EGF R Antibody, Human IgG1 (Cat. No. EGR-M1201) at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with a linear range of 0.002-0.016 ng/mL (QC tested).
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 5 μg/mL (100 μL/well) can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with a linear range of 1-20 ng/mL (Routinely tested).
Immobilized Human EGF R, His Tag (Cat. No. EGR-H5222) at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-1 ng/mL (Routinely tested).
Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Human EGF R, His Tag (Cat. No. EGR-H5222) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.23 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag (Cat. No. EGR-H5222) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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