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Your Position: Home > Receptor Tyrosine Kinases > EGF R > EGR-H5222

Human EGF R Protein, His Tag (MALS verified)

  • Synonym
  • Source
    Human EGF R, His Tag (EGR-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Ser 645) P00533-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 70.5kDa. The protein migrates as 95-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Human EGF R, His Tag (Cat. No. EGR-H5222) SDS-PAGE gel

Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Human EGF R, His Tag (Cat. No. ) MALS images

The purity of Human EGF R, His Tag(Cat. No. EGR-H5222) was more than 90% and the molecular weight of this protein is around 90-115 kDa verified by SEC-MALS.

Human EGF R, His TagHuman EGF R, His Tag (Cat. No. EGR-H5222) ELISA bioactivity

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with a linear range of 0.006-0.025 μg/mL (QC tested).


Erbitux (Cetuximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).


Loaded Erbitux (Cetuximab) on AHC Biosensor, can bind Human EGF R, His Tag (Cat. No. EGR-H5222) with an affinity constant of 1.23 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of Human EGF R, His Tag (Cat. No. EGR-H5222) and negative control protein respectively, washed and then followed by PE anti-His antibody and analyzed with FACS (Routinely tested).

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