Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 48.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD40 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD40 Protein, Fc,Avitag (Cat. No. TN5-H82F9) is more than 90% and the molecular weight of this protein is around 105-120 kDa verified by SEC-MALS.
Immobilized Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD40 Protein, Fc,Avitag (Cat. No. TN5-H82F9) with a linear range of 0.01-0.625 μg/mL (QC tested).
Immobilized Biotinylated Human CD40 Protein, Fc,Avitag (Cat. No. TN5-H82F9) at 5 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Human CD40 Ligand, His,Flag Tag (Cat. No. CDL-H52Db) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
FACS analysis shows that Biotinylated Human CD40, Fc,Avitag (Cat. No. TN5-H82F9) can bind to 293T cells overexpressing human CD40L. The concentration of Biotinylated Human CD40 is 0.1 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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