This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human EGF R, Fc Tag(Cat. No. EGR-H5252) is more than 90% and the molecular weight of this protein is around 230-260 kDa verified by SEC-MALS.
Authors: Meng Wang, Youke Qi, Yongning Sun
Journal: BioMed Research International 2020
Authors: Lobb Roy, et al.
Journal: US20180142035A1 2018
Authors: Jakob W. Buecheler, et al.
Journal: J Chem Technol Bio 2014
Application: Biolayer interferometry (BLI)
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