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Your Position: Home > All Other Proteins > CD4 > CD4-H82F3

Biotinylated Human CD4 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    CD4,CD4mut,LEU3
  • Source
    Biotinylated Human CD4, Fc,Avitag (CD4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).
    Predicted N-terminus: Lys 26
  • Molecular Characterization
    Online(Lys 26 - Pro 396) P01730-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 69.7 kDa. As a result of glycosylation, the protein migrates as 80 kDa under reducing (R) condition, and 150 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) SDS-PAGE gel

Biotinylated Human CD4, Fc,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD4, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) was more than 90% and the molecular weight of this protein is around 170-200 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD4, Fc,AvitagBiotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) ELISA bioactivity

Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) with a linear range of 0.2-6 ng/mL (QC tested).

Biotinylated Human CD4, Fc,AvitagBiotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) ELISA bioactivity

Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3)) with a linear range of 0.5-8 ng/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

2e5 of anti-CD4 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Human CD4, Fc,Avitag (Cat. No. CD4-H82F3) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

  • Background
    T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:Approved

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