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Your Position: Home > Immune Checkpoint Proteins > 2B4 > 2B4-H82E9

Biotinylated Human 2B4 / CD244 / SLAMF4 Protein, Avitag™,His Tag (recommended for biopanning)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD244,2B4,SLAMF4,NKR2B4,NAIL,h2B4
  • Source
    Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (2B4-H82E9) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).
    Predicted N-terminus: Cys 22
  • Molecular Characterization
    Online(Cys 22 - Arg 221) Q9BZW8-2

    This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 25.4 kDa. The protein migrates as 40-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) SDS-PAGE gel

Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning)Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) ELISA bioactivity

Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Fc Tag (Cat. No. BC1-H5255) with a linear range of 0.3-5 ng/mL (QC tested).

Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning)Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) ELISA bioactivity

Immobilized Biotinylated Human 2B4, Avitag,His Tag (recommended for biopanning) (Cat. No. 2B4-H82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human CD48, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BC1-H5258) with a linear range of 0.3-5 ng/mL (Routinely tested).

  • Background
    Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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