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Your Position: Home > Immune Checkpoint Proteins > CD155 > Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag

Mouse CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
  • Source
    Mouse CD155, Mouse IgG2a Fc Tag (CD5-M5254) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Leu 348 (Accession # Q8K094-1).
    Predicted N-terminus: Asp 29
  • Molecular Characterization
    CD155(Asp 29 - Leu 348) Q8K094-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 61.8 kDa. The protein migrates as 35 kDa and 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-M5254) SDS-PAGE gel

Mouse CD155, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse CD155, Mouse IgG2a Fc TagMouse CD155, Mouse IgG2a Fc Tag (Cat. No. CD5-M5254) ELISA bioactivity

Immobilized Mouse TIGIT, His Tag (Cat. No. TIT-M52E6) at 10 μg/mL (100 μL/well) can bind Mouse CD155, mouse IgG2a Fc tag (Cat. No. CD5-M5254) with a linear range of 0.039-1.25 μg/mL (QC tested).

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