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Your Position: Home > Protein > Integrin alpha E beta 7 > IT7-H52W7

Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein, His Tag&Tag Free

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  • Synonym
    Integrin alpha E beta 7,ITGAE&ITGB7
  • Source
    Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free(IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).
    Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)
  • Molecular Characterization
    Integrin alpha E beta 7 Structure

    Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). The protein migrates as 100-115 kDa and 135-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha E beta 7 SDS-PAGE

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-SPR
 Integrin alpha E beta 7 SPR

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W7) immobilized on CM5 Chip can bind Human E-Cadherin, Fc Tag, premium grade (Cat. No. ECD-H5250) with an affinity constant of 0.148 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Integrin alpha E beta 7 consist of two major subunits: Integrin alpha E (ITGAE) also known as CD103 (cluster of differentiation 103) and Integrin beta-7 is an integrin protein that in humans is encoded by the ITGB7 gene. Integrin alpha E beta 7 (CD103) is expressed mainly by cells of the T lymphocyte lineage within mucosal tissues. This is a strikingly narrow pattern of expression compared with that of other integrins. Lymphocytes expressing alpha E beta 7 are abundant in the gut and comprise a major part of the total T cell complement of the body.
    The effectiveness of lung transplantation is marred by the relatively high incidence of rejection. The lung normally contains a large population of lymphocytes in contact with the airway epithelium, a proportion of which expresses the mucosal integrin, alpha(E)(CD103)beta(7). This integrin is not a homing receptor, but is thought to retain lymphocytes at the epithelial surface.
  • Clinical and Translational Updates

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