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Your Position: Home > All Other Proteins > Integrin alpha E beta 7 > IT7-H52W7

Human Integrin alpha E beta 7 (ITGAE&ITGB7) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha E beta 7,ITGAE&ITGB7
  • Source
    Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7) (Accession # P38570-1 (ITGAE) & P26010-1 (ITGB7)).
    Predicted N-terminus: Phe 19 (ITGAE) & Glu 20 (ITGB7)
  • Molecular Characterization
    Online(Phe 19 - Ser 1124 (ITGAE) & Glu 20 - His 723 (ITGB7)) P38570-1 (ITGAE) & P26010-1 (ITGB7)

    Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAE and ITGB7, has a calculated MW of 128.6 kDa (ITGAE) and 81.8 kDa (ITGB7). Subunit ITGAE is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB7 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 135-150 kDa (ITGAE) and 100-115 kDa (ITGB7) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT7-H52W7) SDS-PAGE gel

Human ITGAE&ITGB7 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

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