This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 53.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human PD-L1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) is more than 90% and the molecular weight of this protein is around 120-135 kDa verified by SEC-MALS.
Immobilized Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) with a linear range of 2-156 ng/mL (QC tested).
Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) at 1 μg/mL (5 μL/well) can bind Human PD-1 (Cat. No. PD1-H5221) with a linear range of 0.04-1.25 μg/ml (Routinely tested).
AlphaLISA shows that the binding of Biotinylated Human PD-L1, Fc,Avitag (Cat. No. PDL-H82F2) to Human PD-1 (Cat. No. PD1-H5221) was inhibited by increasing concentration of Opdivo. The IC50 is 0.287 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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