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Product Details
Synonyms
CD4, CD4mut, LEU3
Source
Biotinylated Human CD4, His,Avitag (CD4-H82E8) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).
Predicted N-terminus: Lys 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 45 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human CD4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
Immobilized HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (Cat. No. GP4-V15223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 2-78 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface antigen CD4
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
25 Details
- Highest Development Stage：
Approved
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