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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD4, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) is more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.
Immobilized Ibalizumab at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 0.2-3 ng/mL (QC tested).
Immobilized HIV-1 [HIV-1/Clade C (16055)] GP120, His Tag (Cat. No. GP5-V15224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 1-16 ng/mL (Routinely tested).
Immobilized HIV-1 [HIV-1/Clade E (CM244)] GP120, His Tag (Cat. No. GP4-V15223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD4, His,Avitag (Cat. No. CD4-H82E8) with a linear range of 2-78 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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