This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) is more than 90% and the molecular weight of this protein is around 99-121 kDa verified by SEC-MALS.
Loaded Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 1.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zhao Y, et al
Journal: Immunity 2019
Application: Cell Culture
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