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Your Position: Home > Immune Checkpoint Proteins > CD28 > CD8-H52A5

Human / Cynomolgus / Rhesus macaque CD28 Protein, Mouse IgG2a Fc Tag (MALS verified)

  • Synonym
    CD28,Tp44
  • Source
    Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (CD8-H52A5) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
    Predicted N-terminus: Asn 19
  • Molecular Characterization
    Online(Asn 19 - Pro 152) P10747-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) SDS-PAGE gel

Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. ) MALS images

The purity of Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) was more than 90% and the molecular weight of this protein is around 99-121 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc TagHuman / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) ELISA bioactivity

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag (Cat. No. CD6-H5257) with a linear range of 0.1-5 μg/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag (Cat. No. CD8-H52A5) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 1.66 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Citations
  • Background
    T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:12 Details
  • Latest Research Phase:Phase 3 Clinical

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