Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 110.7 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGA5) and 100-120 kDa (ITGB1) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human ITGA5&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Authors: Takada, Yoko K et al.
Journal: The Journal of biological chemistry 2021
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ACROBiosystems has developed an exclusive immune checkpoint protein collection， The consistency and excellent performance of our proteins are confirmed by a variety of assays.
ACROBiosystems has developed a series of homogeneous CD3 proteins with good bioactivity. They are verified as 1:1 heterodimers by nonreductive electrophoresis and MALS as well.
ACROBiosystems has developed a series of products with different species and tags for a variety of hot targets. It is suitable for immune, antibody screening, SPR, cell activity detection and other experiments, and all SPR&BLI protocols are available for free.
We also supply difficult-to-express proteins such as BCMA, CD19, ROR1, and EGFRVIII.
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