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Your Position: Home > Immune Checkpoint Proteins > LILRA5 > LI5-H82E1

Biotinylated Human LILRA5 / CD85f / ILT11 Protein, His,Avitag™

  • Synonym
  • Source
    Biotinylated Human LILRA5, His,Avitag (LI5-H82E1) is expressed from human 293 cells (HEK293). It contains AA Gly 42 - Arg 268 (Accession # A6NI73-1).
    Predicted N-terminus: Gly 42
  • Molecular Characterization
    Online(Gly 42 - Arg 268) A6NI73-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 28.9 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) SDS-PAGE gel

Biotinylated Human LILRA5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Biotinylated Human LILRA5, His,AvitagBiotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) ELISA bioactivity

Immobilized Human ANGPTL7, His Tag (Cat. No. AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LILRA5, His,Avitag (Cat. No. LI5-H82E1) with a linear range of 1.25-20 μg/mL (QC tested).

  • Background
    LIRs are immunoglobulin-like receptors that have activating and inhibitory functions in leukocytes. Leukocyte immunoglobulin-like receptor A5 (LILRA5) belongs to a family of receptors known to regulate leukocyte activation. LILRA5, expressed as a membrane-bound receptor and as a secreted molecule. The transmembrane LILRA5 contain a short cytoplasmic domain and a charged arginine residue within the transmembrane region that is likely to mediate its association with another coreceptor. LILRA5 is mostly expressed in myeloid cells, including monocytes and neutrophils.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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