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Your Position: Home > Immune Checkpoint Proteins > CD27 > Cynomolgus / Rhesus macaque CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

Cynomolgus / Rhesus macaque CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxin (CD7-C5259) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Arg 191 (Accession # XP_005569963.1). In the region Ala 20 - Arg 191, the AA sequence of Cynomolgus and Rhesus macaque CD27 are homologus.
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    Online(Ala 20 - Arg 191) XP_005569963.1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-C5259) SDS-PAGE gel

Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxinCynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-C5259) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) with a linear range of 2-31 ng/mL (QC tested).

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