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Your Position: Home > Protein > CD27 > CD7-C5259

Cynomolgus / Rhesus macaque CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (CD7-C5259) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Arg 191 (Accession # XP_005569963.1). In the region Ala 20 - Arg 191, the AA sequence of Cynomolgus and Rhesus macaque CD27 are homologus.
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    Online(Ala 20 - Arg 191) XP_005569963.1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 46.2 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) SDS-PAGE gel

Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc TagCynomolgus / Rhesus macaque CD27 Protein, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) ELISA bioactivity

Immobilized Cynomolgus / Rhesus macaque CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-C5259) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) with a linear range of 2-31 ng/mL (QC tested).

  • Background
    CD Antigen CD27 is also known as Tumor necrosis factor receptor superfamily member 7 (TNFRSF7), which belongs to TNF-receptor superfamily. CD27 / TNFRSF7 is found in most T-lymphocytes. CD27 / TNFRSF7 is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-κB and MAPK8/JNK. CD27 has been shown to interact with SIVA1, TRAF2 and TRAF3.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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