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Product Details
Synonyms
TSLP
Source
Biotinylated Human TSLP, His,Avitag (TSP-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).
Predicted N-terminus: Tyr 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 18.6 kDa. The protein migrates as 22-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
0.7-1.0 as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>85% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human TSLP, His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) is more than 85% and the molecular weight of this protein is around 23-33 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 4-31 ng/mL (QC tested).
Protocol
Immobilized Anti-TSLP MAb, Human IgG2 at 2 μg/mL (100 μL/well) can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with a linear range of 0.1-2 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) at 2 μg/mL (100 μL/well) via precoated 5 μg/mL (100 μL/well) of Human TSLP R, Fc Tag (Cat. No. TSR-H525a), can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Serial dilutions of Monoclonal Anti-Human TSLP Antibody, Human IgG2 were added into Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H525x) : Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) binding reactions. The half maximal inhibitory concentration (IC50) is 0.21 μg/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) on SA Biosensor, can bind Monoclonal Anti-Human TSLP Antibody, Human IgG2 with an affinity constant of 15.2 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human TSLP R, Fc Tag (Cat. No. TSR-H525a) on Protein A Biosensor, can bind Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) with an affinity constant of 13.2 nM as determined in BLI assay (ForteBio Octet R8)(Routinely tested).
Protocol
Bioactivity-Flow Cytometry
2e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1 μg/mL of Biotinylated Human TSLP Protein, His,Avitag (Cat. No. TSP-H82Eb) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
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