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Your Position: Home > Recombinant Proteins > BCMA > Human BCMA / TNFRSF17 Protein, Llama IgG2b Fc Tag, low endotoxin

Human BCMA / TNFRSF17 Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Human BCMA, Llama IgG2b Fc Tag (BCA-H5259) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    BCMA(Met 1 - Ala 54) Q02223-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 33.7 kDa. The protein migrates as 40-47 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) SDS-PAGE gel

Human BCMA, Llama IgG2b Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity
Human BCMA, Llama IgG2b Fc TagHuman BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) ELISA bioactivity

Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 5 μg/mL (100 μL/well) can bind Human BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) with a linear range of 0.02-0.4 ng/mL (QC tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

Human BCMA, Llama IgG2b Fc TagHuman BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) ELISA bioactivity

Immobilized Human BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.4-3 ng/mL (Routinely tested).

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