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Your Position: Home > All Other Proteins > CD52 > Human CD52 Protein, Fc Tag

Human CD52 Protein, Fc Tag

  • Synonym
    CDW52,HE5,CD52,CAMPATH-1 antigen
  • Source
    Human CD52, Fc Tag (CD2-H525a) is expressed from human 293 cells (HEK293). It contains AA Gly 25  - Ser 36 (Accession # P31358-1).
    Predicted N-terminus: Gly 25
  • Molecular Characterization
    CD52(Gly 25  - Ser 36) P31358-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 27.9 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CD52, Fc Tag (Cat. No. CD2-H525a) SDS-PAGE gel

Human CD52, Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Human CD52, Fc TagHuman CD52, Fc Tag (Cat. No. CD2-H525a) ELISA bioactivity

Immobilized Human CD52, Fc Tag (Cat. No. CD2-H525a) at 10 μg/mL (100 μL/well) can bind Alemtuzumab with a linear range of 0.313-10 μg/mL (QC tested).

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