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Your Position: Home > Immune Checkpoint Proteins > CD27 > Human CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Human CD27, Mouse IgG2a Fc Tag, low endotoxin (CD7-H5257) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Ile 192 (Accession # P26842-1).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    Online(Thr 21 - Ile 192) P26842-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 46.2 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) SDS-PAGE gel

Human CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD27, Mouse IgG2a Fc Tag, low endotoxinHuman CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) ELISA bioactivity

Immobilized Human CD27 Ligand, Fc Tag (Cat. No. TN7-H526x) at 1 μg/mL (100 μL/well) can bind Human CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-H5257) with a linear range of 0.005-0.078 μg/mL (QC tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Flow Cytometry assay shows that Human CD27, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD7-H5257) can bind to Raji cells Surface Human CD70. The concentration of CD27 used is 10 μg/mL (Routinely tested).

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Price(USD) : $350.00

Price(USD) : $2310.00

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