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Your Position: Home > Recombinant Proteins > CD27 > Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse CD27 / TNFRSF7 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CD27,TNFRSF7,S152,T14,Tp55
  • Source
    Mouse CD27, Mouse IgG2a Fc Tag (CD7-M5252) is expressed from human 293 cells (HEK293). It contains AA Thr 21 - Arg 182 (Accession # P41272-1).
    Predicted N-terminus: Thr 21
  • Molecular Characterization
    CD27(Thr 21 - Arg 182) P41272-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) SDS-PAGE gel

Mouse CD27, Mouse IgG2a Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse CD27, Mouse IgG2a Fc TagMouse CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) ELISA bioactivity

Immobilized Mouse CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL (QC tested).

Mouse CD27, Mouse IgG2a Fc TagMouse CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) ELISA bioactivity

Immobilized Mouse CD27, Mouse IgG2a Fc Tag (Cat. No. CD7-M5252) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag (Cat. No. TN7-H82F4) with a linear range of 1-16 ng/mL (Routinely tested).

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