This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 59.2 kDa respectively. The protein migrates as 17 kDa and 65-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human PCSK9, His Tag (Cat. No. PC9-H5223) was more than 90% and the molecular weight of this protein is around 70-80 kDa verified by SEC-MALS.
Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 2.17 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded PCSK9 Mab, Human IgG1 on Protein A Biosensor, can bind Human PCSK9, His Tag (Cat. No. PC9-H5223) with an affinity constant of 88.6 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Yuan J, Cai T, Zheng X, et al.
Journal: Protein Cell 2021
Application: Flow cytometry
Authors: M Wijers, et al
Journal: JCI Insight 2019
Application: Tissue culture
Authors: Lichtor PA, et al
Journal: Nat Chem Biol 2019
Authors: J Rohloff et al.
Journal: US9938314B2 2018
Authors: Grin PM, et al.
Journal: Sci Rep 2018
Application: Cell Culture
Authors: Dugardin C, et al.
Journal: Acta Diabetol 2016
Application: Cell culture
Authors: Topchiy E, et al.
Journal: PLoS One 2016
Authors: Boyd JH, et al.
Journal: Journal of Innate Immunity 2016
Authors: Walley KR, et al.
Journal: Sci Transl Med 2014
Authors: Ledbetter, et al.
Journal: US20140120091A1 2014
Application: Capture ELISA
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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