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Your Position: Home > Immune Checkpoint Proteins > CD40 > CD0-H525a

Human CD40 / TNFRSF5 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

  • Synonym
    CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50
  • Source
    Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) (CD0-H525a) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Arg 193 (Accession # P25942-1).
    Predicted N-terminus: Glu 21
  • Molecular Characterization
    Online(Glu 21 - Arg 193) P25942-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 46.1 kDa. The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) (Cat. No. CD0-H525a) SDS-PAGE gel

Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) (Cat. No. ) MALS images

The purity of Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) (Cat. No.CD0-H525a) was more than 90% and the molecular weight of this protein is around 110-125 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)Human CD40, Mouse IgG2a Fc Tag, low endotoxin (MALS verified) (Cat. No. CD0-H525a) ELISA bioactivity

Immobilized Human CD40 Ligand, Fc Tag (Cat. No. CDL-H5267) at 5 μg/mL (100 μL/well) can bind Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) with a linear range of 20-156 ng/mL (QC tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) on Protein A Biosensor, can bind Human CD40 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Db) with an affinity constant of 0.911 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that Human CD40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD0-H525a) can bind to 293T cells overexpressing human CD40L. The concentration of Human CD40 is 0.03 μg/ml (Routinely tested).

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Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

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