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Your Position: Home > Immune Checkpoint Proteins > OX40 > Human OX40 / TNFRSF4 / CD134 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human OX40 / TNFRSF4 / CD134 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    TNFRSF4,OX40,CD134,OX40L receptor,ACT35,TXGP1L
  • Source
    Human OX40, Mouse IgG2a Fc Tag, low endotoxin (OX0-H5252) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Ala 216 (Accession # P43489-1).
    Predicted N-terminus: Leu 29
  • Molecular Characterization
    OX40(Leu 29 - Ala 216) P43489-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 47.1 kDa. The protein migrates as 58-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human OX40, Mouse IgG2a Fc Tag, low endotoxin  (Cat. No. OX0-H5252) SDS-PAGE gel

Human OX40, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human OX40, Mouse IgG2a Fc Tag, low endotoxin Human OX40, Mouse IgG2a Fc Tag, low endotoxin  (Cat. No. OX0-H5252) ELISA bioactivity

Immobilized Human OX40 Ligand, His Tag (Cat. No. OXL-H52Q8) at 2 μg/mL (100 μL/well) can bind Human OX40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. OX0-H5252) with a linear range of 0.78-25 ng/mL (QC tested).

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Human OX40, Mouse IgG2a Fc Tag (Cat. No. OX0-H5252) can bind to 293T cell overexpressing human OX40L. The concentration of OX40 is 0.01 μg/ml (Routinely tested).

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