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Product Details
Synonyms
TACSTD2, GA733-1, M1S1, TROP2
Source
Human TROP-2, His Tag (TR2-H5223) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Thr 274 (Accession # P09758-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Mass Spectrometry
Measured Molecular Mass
The molecular weight by mass spectrometry is 29371 Da (Routinely tested).
Sequence Coverage
Human TROP-2, His Tag(TR2-H5223) was digested with trypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
N-terminal Sequence Analysis
Gln‑Asp‑Asn‑Cys‑Thr‑Cys‑Pro‑Thr‑Asn‑Lys‑Met‑Thr‑Val‑Cys‑Ser
(Routinely tested)
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human TROP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human TROP-2, His Tag (Cat. No. TR2-H5223) is more than 95% and the molecular weight of this protein is around 33-50 kDa verified by SEC-MALS.
Report
Mass Spectrometry
ESI-MS analysis of Human TROP-2, His Tag (Cat. No. TR2-H5223), showing a major peak at 29371 Da. The first Q residue undergoes pyroglutamate formation, resulting in a mass decrease of 17 Da. The minor peak following the major peak is a matrix-associated artifact of the ESI-MS (Routinely tested).
Bioactivity-ELISA
Immobilized Human TROP-2, His Tag (Cat. No. TR2-H5223) at 1 μg/mL (100 μL/well) can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Monoclonal Anti-His Tag Antibody Europium chelate (for TR-FRET) (Cat. No. FRT-TD05) is used as the Donor and Monoclonal Anti-Human IgG Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA02) is used as the Acceptor. Human TROP-2, His Tag (Cat. No. TR2-H5223), at 1.25 ng/well, can bind to the Datopotamab with a linear detection range of 8-0.001 μg/mL. (Routinely tested)
Bioactivity-SPR
Captured Trop2 antibody on CM5 chip via anti-mouse antibodies surface can bind Human TROP-2, His Tag (Cat. No. TR2-H5223) with an affinity constant of 5.98 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
V***w2023-02-21
LikesAcro and their customer support are super helpful. Products ordered arrived in a timely manner. Customer support personal are as friendly as they are insightful. Never have they disappointed when purchasing any of their products. Such a user friendly website and if anything is not found customer support never backs down to help out! Acro’s personal are very friendly and knowledgeable. They were friendly and helpful when resolving this issue.- E***l2022-10-26 LikesAcro’s customer support is always super helpful and quick to respond. When products are ordered, the arrive in a timely manner. Acro’s personal are very friendly and knowledgeable. They never disappoint when it comes to finding a solution to a problem. Having such a user-friendly website helps a lot in finding the right product needed and if something cannot be found customer support always finds a way!
1***32022-08-10 LikesACRO's antigens are well solubilized and encapsulated for antibody screening, and the method is now being established smoothly. Also, they provide us with complete technical support which can be given in various aspects for any problems that arise during the test.
FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Tumor-associated calcium signal transducer 2
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
47 Details
- Highest Development Stage：
Approved
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