This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 33.1 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human EGF, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human EGF R, His Tag (Cat. No. EGR-H5222) captured on CM5 Chip via anti-His antibody can bind Human EGF, Mouse IgG2a Fc Tag (Cat. No. EGF-H525b) with an affinity constant of 2.63 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Authors: Wang Z, Hao Z, Yu S, et al.
Journal: NANOMATERIALS-BASEL 2020
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