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Your Position: Home > Receptor Tyrosine Kinases > VEGF R3 > Mouse VEGF R3 / FLT4 Protein, Mouse IgG2a Fc Tag, low endotoxin

Mouse VEGF R3 / FLT4 Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FLT-41,FLT41,PCL
  • Source
    Mouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxin (FL4-M5251) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Asp 770 (Accession # P35917-1).
    Predicted N-terminus: Tyr 25
  • Molecular Characterization
    VEGF R3(Tyr 25 - Asp 770) P35917-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 111.5 kDa. The protein migrates as 116-125 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Mouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. FL4-M5251) SDS-PAGE gel

Mouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity
Mouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxinMouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. FL4-M5251) ELISA bioactivity

Immobilized Human VEGF-C, His Tag (Cat. No. VEC-H4225) at 5 μg/mL (100 μL/well) can bind Mouse VEGF R3, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. FL4-M5251) with a linear range of 1-10 ng/mL (QC tested).

  • Background
    Vascular endothelial growth factor receptor 3 (VEGF R3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGF R3 mediates lymphangiogenesis in response to VEGF-C and VEGF-D. VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.
  • References
  • Limited Use License

    The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent. The purchase of the Acrobiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.

    Commercial use of the Avitag™ technology to manufacture a commercial product, or use of the Avitag™ technology to facilitate or advance research which will be applied to the development of a commercial product requires a license from Avidity, LLC. Examples of Commercial use include, but are not limited to biosensors, diagnostics, therapeutic applications, proximity assays, and drug screening assays.

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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